486
OPTION A: attaching the adapter to the V-T phototube of the side port
OPTION B: attaching the adapter directly to the tube opening
The following considerations need to be taken into account:
New LM Digital Adapter for:Sony Alpha 9 II (ILCE-9M2) / Sony Alpha 9 / Nikon D6 / Sony Alpha 7R IV / Sony Alpha 7S II / Sony Alpha 7R III / Nikon Z6 / Sony Alpha 7R II / Nikon Z7 / Canon EOS R with Canon Adapter EF-EOS R / Canon EOS Ra (Astro) with Canon Adapter EF-EOS R / Canon EOS RP with Canon Adapter EF-EOS R / Sony Alpha 7S / Canon EOS 1D X Mark III / Nikon Z50 / Nikon D850 / Canon EOS 1D X Mark II / Nikon D780 / Sony Alpha 7III / Nikon D5 / Sony Alpha 6600 / Sony Alpha 6400 / Sony Alpha 6100 / Canon EOS 1D X / Nikon D4s / Olympus OM-D E-M5 III / Canon EOS 90D / Canon EOS 5D Mark IV / Nikon D4 / Nikon D750 / Canon EOS 6D Mark II / Fujifilm X-T4 / Fujifilm X-T3 / Sony Alpha 6300 / Sony Alpha 6500 / Nikon D500 / Nikon D810 / Nikon D800 / Canon EOS M6 Mark II / Nikon D800E / Nikon Df / Panasonic Lumix DC-G9 / Nikon D610 / Nikon D600 / Canon EOS 250D / Canon EOS 850D / Rebel T8i / Canon EOS 6D / Sony Alpha 99 II (SLT-A99 II) / Canon EOS M200 / Canon EOS 5DS R ( without low-pass filter) / Olympus OM-D E-M1 Mark II /
This adapter communicates to all the devices on the Ti microscope through the TIScope hub device. Thus, to use any of these devices, you will need to first add the TIScope device.
- Nikon Eclipse E200 User Manual
- Nikon Eclipse Ti-u Manual
- Nikon Eclipse Ti Manual Pdf
- Nikon Eclipse Ti Confocal Manual
- Nikon Eclipse Ts100 Manual
At the center of it all is the Eclipse Ti-E, a powerful inverted microscope system that provides instant access to all of these techniques plus revolutionary Nikon optics. Elementary Numerical Analysis Atkinson Solutions Manual. Yamaha Xj6 Manual 2018 Victory Vegas Owners Manual. Of today’s high-end research, Nikon has developed the new Ti series of microscopes. Combined with NIS-Elements imaging software, the Ti supports diverse image acquisition and analysis methods such as multi-dimensional time-lapse imaging to acquire temporal, spatial and spectral information of fast, dynamic live cell processes. In combination with Nikon's powerful acquisition and analysis software, NIS-Elements, the Ti2 is a total innovation in imaging. Download Eclipse Ti2 Brochure (12.6MB) Download Eclipse. At the center of it all is the Eclipse Ti Series, a powerful series of inverted microscopes that provide access to all of these techniques plus revolutionary Nikon CFI60 optics. The Eclipse Ti Series, combined with Nikon's NIS-Elements imaging software, enables you to generate data faster, more easily, and more accurately than ever before.
Nikon’s Eclipse TE200/TE300 inverted microscopes are suitable for use in a broad spectrum of application fields. The two models were developed in the late 1990s but are still a good choice for photomicrography applications when combined with our LM digital adapters with plan achromatic precision optics. The TE300 even features a front port, which the lower-priced TE200 doesn’t have. Both models feature a side port, which can be used to attach a DSLR camera, mirrorless system camera or C-mount camera to the microscope.
Illumination with a 30W halogen lamp is standard on the TE200; however, this can be replaced (similar to the TE300) with a 100W illumination pillar. Depending on the model, the light distribution ratio is switchable between 100:0, 20:80 and 0:100.
Jul 08, 2018 At the center of it all is the Eclipse Ti-E, a powerful inverted microscope system that provides instant access to all of these techniques plus revolutionary Nikon optics. Elementary Numerical Analysis Atkinson Solutions Manual. Yamaha Xj6 Manual 2018 Victory Vegas Owners Manual.
Both models in the series feature Nikon’s powerful infinity CFI60 optical system. The CFI60 lenses have a parfocal distance of 60 mm, a 25 mm thread size and a 22 mm field of view.
Choice of objectives:
- Achromats
- Plan Fluors
- Plan Fluors with extra-long working distance
- Plan Apochromats
- Hoffman modulation contrast objectives
The well-balanced design of the TE200 and TE300 offers high optical performance, combined with convenient controls for comfortable, fatigue-free operation. In addition to standard observation modes (bright field, dark field and phase contrast), observation techniques such as epi-fluorescence, Hoffman modulation contrast or Nomarski DIC are supported as well. Differential interference contrast (DIC) is a valuable tool for imaging unstained transparent live organisms with high contrast. For cell cultures grown in plastic Petri dishes, the Hoffmann modulation contrast method can be used to produce images with a 3D appearance.
The design is highly modular and provides options for the NT-88NE micromanipulator system, the MHW water-hydraulic micromanipulator for electrophysiology and patchclamp research, an incubator box, discussion equipment, the FX-III series photomicrographic system, a mechanical stage, plate holder, etc.
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The sextuple revolving nosepiece of the E600 and E800 microscopes is also compatible with the TE200 and TE300 microscopes. Two mounting options are available: attaching the adapter to the V-T phototube of the side port (option A with 42 mm inner diameter) or removing the V-T phototube and attaching the adapter directly to the tube opening (option B with 38 mm inner diameter). The diagrams below illustrate the two connection methods:
OPTION A: attaching the adapter to the V-T phototube of the side port
OPTION B: attaching the adapter directly to the tube opening
Available eyepiece tubes are the TE-TL and TE-TS tubes (both are binocular tubes). An intermediate tube (TE-TI) is required for attaching the usual Nikon trinocular tubes (Y-TF and Y-TT). The key to properly attaching a DSLR or system camera to these tubes with our high-quality LM digital adapters is to choose the correct combination:
The following considerations need to be taken into account:
- Does the tube have a Nikon YT-TV adapter (shorter) or a Nikon V-T photo adapter (longer)? These are essential for attaching a camera to the microscope and cannot be bypassed!
- The Nikon YT-TV adapter accepts either the TUST38C LM C-mount adapter or – if you already have one – the Nikon C-mount adapter A (1x). The LM digital adapter, which is connected to the camera, is then added to that combination.
- The LM DSLR adapter is directly attached to the Nikon V-T photo adapter.
- If the microscope doesn’t have a phototube, the camera can also be attached to the eyepiece tube. The image quality is as good as that achieved when attaching the camera to the phototube, and the adapter solution for the eyepiece tube is universal, because it enables compatibility with a wide range of different microscopes. We offer a comprehensive range of products for a large variety of different camera systems.
Conclusion: Equipped with the renowned CFI60 optical system, Nikon’s Eclipse TE200 and TE300 models are very well suited for digital photomicrography.
Photography:
Fitting the microscope to digital single-lens reflex (DSLR), system or C-mount cameras is easy with our LM digital SLR adapters, which feature a plan achromatic optical system. Our products make it possible to capture top-quality microscope images. To help you select the adapter that is right for your camera, we have set up an online configurator on our website. You can also email us – ideally with attached photographs of your microscope.
Modern DSLR and system cameras offer the latest technology and are generally very well suited for microscopy applications. Most of them can be controlled remotely via PC/Mac. Because of their high sales volumes, they offer an excellent price/performance ratio compared to special-purpose microscope cameras.
Features of top DSLR and system cameras:
- Large, powerful full-frame sensors (36 x 24 mm)
- Sensor resolution of 50 megapixels
- High light sensitivity (ISO 100,000+)
- Extensive dynamic range (up to 14 aperture stops/f-stops)
- Short exposure times (1/8000 second):
- Full/Ultra HD (4k) video function
- Live video capture on external monitors in ultra HD quality
- Large, powerful full-frame sensors (36 x 24 mm)
- Sensor resolution of 50 megapixels
- High light sensitivity (ISO 100,000+)
- Extensive dynamic range (up to 14 aperture stops/f-stops)
- Short exposure times (1/8000 second):
- Full/Ultra HD (4k) video function
- Live video capture on external monitors in ultra HD quality
In most cases, these cameras are significantly more powerful than microscope cameras with smaller sensors (1/2' or 2/3'). On our website you will find our current camera recommendations and a camera ranking which is specifically tailored to microscopy applications.
New LM Digital Adapter for:Sony Alpha 9 II (ILCE-9M2) / Sony Alpha 9 / Nikon D6 / Sony Alpha 7R IV / Sony Alpha 7S II / Sony Alpha 7R III / Nikon Z6 / Sony Alpha 7R II / Nikon Z7 / Canon EOS R with Canon Adapter EF-EOS R / Canon EOS Ra (Astro) with Canon Adapter EF-EOS R / Canon EOS RP with Canon Adapter EF-EOS R / Sony Alpha 7S / Canon EOS 1D X Mark III / Nikon Z50 / Nikon D850 / Canon EOS 1D X Mark II / Nikon D780 / Sony Alpha 7III / Nikon D5 / Sony Alpha 6600 / Sony Alpha 6400 / Sony Alpha 6100 / Canon EOS 1D X / Nikon D4s / Olympus OM-D E-M5 III / Canon EOS 90D / Canon EOS 5D Mark IV / Nikon D4 / Nikon D750 / Canon EOS 6D Mark II / Fujifilm X-T4 / Fujifilm X-T3 / Sony Alpha 6300 / Sony Alpha 6500 / Nikon D500 / Nikon D810 / Nikon D800 / Canon EOS M6 Mark II / Nikon D800E / Nikon Df / Panasonic Lumix DC-G9 / Nikon D610 / Nikon D600 / Canon EOS 250D / Canon EOS 850D / Rebel T8i / Canon EOS 6D / Sony Alpha 99 II (SLT-A99 II) / Canon EOS M200 / Canon EOS 5DS R ( without low-pass filter) / Olympus OM-D E-M1 Mark II /
- 3Device-specific notes
Nikon Ti motorized microscope
Summary: | Adapter for Nikon Ti motorized microscopes |
Author: | Nico Stuurman and Roy Wollman Karl Hoover (additional accessory devices, Matlab compatibility) Mark Tsuchida |
Maintainer: | Mark Tsuchida |
License: | Source code cannot be made available |
Platforms: | Windows 32-bit and 64-bit (uses driver supplied by Nikon) |
Devices: | Microscope hub (TIScope), nosepiece (TINosePiece), condenser turret (TICondenserCassette), shutters (TIDiaShutter, TIEpiShutter and TIAuxShutter), optical path (TILightPath), reflector turrets (TIFilterBlock1 and -2), filter wheels (TIExcitationFilterWheel and TIBarrierFilterWheel), focus (TIZDrive), PerfectFocus (TIPFSStatus and TIPFSOffset), XY stage (TIXYDrive), halogen lamp (TIDiaLamp), TIRF attachment (TITIRF) |
Since version | 1.2.18; major update in 1.4.15 |
Peripheral device discovery: | Supported |
This adapter communicates to all the devices on the Ti microscope through the TIScope hub device. Thus, to use any of these devices, you will need to first add the TIScope device.
Installation
Do not plug the Ti microscope into a USB 3 port (usually colored blue). The scope may not report its status correctly to the PC after manual operations (observed at least with firmware 5.00, driver 4.3.2.622 or 4.4.1.714). Use a USB 2 port.
This adapter uses the driver and API supplied by Nikon. The latest version of Ti Setup Tool (which includes drivers for the Ti microscope and TiControl, a control and configuration utility) can be downloaded from the Nikon website.
The required Nikon driver and interface libraries (a.k.a. the redistributable components of the Nikon Ti SDK) can also be installed using our own installer:
SDK Version | 32-bit Installer | 32-bit Driver Version | 64-bit Installer | 64-bit Driver Version |
---|---|---|---|---|
4.2.3.572 | N/A | 2.0.2.1 | N/A | 2.0.2.1 |
4.3.2.622 | N/A | 2.0.2.1 | N/A | 2.0.2.2 |
4.3.3.628 | N/A | 2.0.2.1 | N/A | 2.0.2.2 |
4.4.1.672 | 32-bit 4.4.1.672 (updated July 3, 2013) | 2.0.2.1 | 64-bit 4.4.1.672 (updated July 3, 2013) | 2.0.5.1 |
4.4.1.714 | 32-bit 4.4.1.714 (uploaded July 16, 2013) | 2.0.5.1 | 64-bit 4.4.1.714 (uploaded July 16, 2013) | 2.0.5.1 |
After installing one of these, you might need to tell Windows to use the new driver by opening the Windows Device Manager, finding the Nikon Ti microscope (under Imaging Devices), and choosing Properties from the right-click menu. Click Update Driver and tell Windows to search C:Program FilesNikonSharedDrivers (or the appropriate equivalent on your system). Note that the driver version number differs from the SDK and library (DLL) version numbers (the main DLL is normally installed at C:Program FilesNikonSharedBinNikonTi.dll).
To uninstall any of these packages, go to the Programs and Features control panel; the package should be listed with Micro-Manager as Publisher and you can right-click to uninstall.
It is normally okay to install both Nikon's Ti Setup Tool and our TiSDKRedist package. The newer DLLs and drivers will be used by both Micro-Manager and TiControl. To revert to an older version of the driver and DLLs, you will need to uninstall all the packages that include them (our TiSDKRedist, Ti Setup Tool, NIS-Elements, etc.).
Another good way to be able to revert to a previous version is to create a restore point in Windows before installing the new version. This can be done on Windows 7 in Control Panel > System > Advanced system settings > System Protection (or search for 'create a restore point' in the Control Panel search box). This will allow you to undo the installation (and all software installations/removals following that point).
Device-specific notes
There has been a major update to the NikonTI device adapter in version 1.4.15 (nightly build of 20130719). The notes below apply to the new version, unless otherwise noted.
Turret devices
In our experience, movement of the dichroic turret (Filter Block Cassette) causes significant vibrations in the microscope. This results in blurry images. To avoid this, add a delay (step 4 of the hardware configuration wizard) to the FilterBlock device. A delay of 250 msec seems to work well.
Note that for the turret devices in the NikonTI adapter, Delay is measured starting when the movement has completed.
The ExtraDelayMs property defaults to the Delay set in the Hardware Configuration Wizard, and allows you to experiment with different delays without going through the Wizard. Unlike the Delay set in the Wizard, the value is not automatically saved, though.
Shutters
When the Delay is set to zero, the device is considered busy until the driver reports back that the movement has completed. When Delay is greater than zero, the report from the driver is ignored and a fixed delay is used (measured from the start of the movement).
Halogen lamp
With some versions of the Nikon drivers, the TIDiaLamp control may be unstable (may cause Micro-Manager to crash). If you are having issues, you might want to try and configure without the DiaLamp.
Nikon Eclipse E200 User Manual
The TIDiaLamp device is a shutter device (although it will only make sense to use it as a shutter when doing time-lapse imaging with an extremely long interval).
There is a ComputerControl property, which needs to be On in order to control the lamp. Lamp intensities set through manual control and through computer control are independent of each other.
The Delay setting works similarly to the shutters, but also applies when setting the Intensity.
PerfectFocus (PFS)
The valid range for Position depends on the objective in use, even though the apparent range is fixed. The units are not micrometers. For historical reasons, the Position values used by Micro-Manager are 1/40 of the raw steps reported by the Nikon driver.
Analyzer
The TIAnalyzer device is available when there is an analyzer in one of the reflector turrets (Filter Block Cassettes), and simply rotates the turret. We recommend using the TIFilterBlock devices instead.
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IntensiLight
It is possible to control the IntensiLight epi-fluorescence light source shutter. If you want to control the IntensiLight with Micro-Manager, you can either connect it directly to the computer, or you can attach it to the Nikon Ti controller. In the latter case, you will need to go into TiControl -> setting, click on 'Connection', select the left most icon, set the connected shutter to IntensiLight and type to Epi (I had mine attached to shutter 2). Now, you can control the shutter with the Epi Shutter button on the microscope. You can now use the EpiShutter Nikon Ti device in Micro-Manager to control the IntensiLight shutter. Note that you will not be able to control the ND filters this way.
Tube Lens
Nikon Eclipse Ti-u Manual
The manual tube lens switcher of the Nikon Ti is not encoded (the computer has no way of knowing its state). You can encode the tube lens switcher yourself following these instructions: Encoding_the_Nikon_TI_Eclipse_tube_lens.
How to report problems
It is not unusual for bugs to depend on the version of Nikon software (driver and libraries) and microscope firmware. When reporting problems with the NikonTI adapter, please include, in addition to the Micro-Manager and Windows version, the Nikon firmware, driver, and software versions.
Version information is displayed in the Device/Property Browser, under the TIScope device (check 'Show read-only properties'). If you can't get the device to load, the CoreLog (with debug logging enabled) may contain the driver and software versions.
Nikon Eclipse Ti Manual Pdf
The driver version can also be checked in the Windows Device Manager (The Ti microscope should be listed under Imaging Devices, if correctly connected and installed).
Nikon Eclipse Ti Confocal Manual
The Nikon software version is typically the version of the file C:Program FilesNikonSharedBinNikonTi.dll.
Nikon Eclipse Ts100 Manual
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